Dental Professionals - Summary of Research papers

Novamin and Bioactive Glass

Novamin and Bioactive Glass are the main active ingredients of SensiShield Toothpaste

The following abstracts are from research papers published in Dental Journals or presented at IADR and AADR meetings, appertaining to the efficacy of Novamin. present in SensiShield toothpaste.

IADR Abstract

Originally Presented at

82nd General SessionMarch 2004, Hawaii USA

Abstract 1586

In-Vitro Antimicrobial Properties of a Bioactive Glass (NovaMin®) Containing Dentifrice

D.C. Greenspan1, A. E. Clark2, and G.P. LaTorre1

1 NovaMin Technology, Inc., Alachua, FL USA 2 University of Florida, Gainesville, FL USA

Objective:The objective of this study was to determine the antimicrobial properties of a bioactive glass (NovaMin®) containing dentifrice against a number of common oral pathogens, and to compare these to a commercial fluoride dentifrice.

Methods:S. mutans (ATCC #25175), S. sanguinis (ATCC #10556), F. nucleatum (ATCC #10953) and A. neaslundii (ATCC #1903 9) were used in this study. The bacterial were grown in DE broth to a concentration of at least 10*6 CFU/ml. A non-aqueous base (glycerin) was used to formulate the bioactive glass containing dentifrices. NovaMin® was added in either 3% w/w or 10% w/w. A commercial, fluoride dentifrice was used as a control. The test articles were diluted 1:3 in distilled water, and the bacterial colonies were inoculated with the test articles. Two minutes after inoculation, aliquots were taken and plated on Agar for 3 to 7 days, depending on the organism to be counted. Viable CFU’s were visually counted. All experiments presented represent the average of three replicates.

Results:The log reduction in viable CFU’s for the NovaMin® containing dentifrices (both3% and 10% w/w) and NovaMin® powder ranged from 4.5 log against F. nucleatum to 8.3 log against S. sanguinis. Log reduction of CFU’s against A. naeslundii was 6.0 log for the NovaMin® dentifrices, and 6.6 log for the NovaMin® powder. By comparison, the log reduction in CFU from the commercial fluoride dentifrice was only 1.1 log against A. naesuundii, 1.3 against F. nucleatum, 1.4 against S. mutans and 3.0 against S. Sanguinis. All three NovaMin® formulations were significantly more effective at reducing bacterial count than the commercial fluoride dentifrice.

Conclusion:These tests demonstrate a strong anti-microbial effect of bioactive glass in a dentifrice formulation. These compounds may prove useful in maintaining optimal oral health.

Evaluation of NovaMin® as an adjunct to fluoride for caries lesion remineralization.

NovaMin Research Report

Research Institutions:

University of Florida Indiana University/.Purdue University at Indianapolis

Investigators:Samuel Shirier Alaudin, MSMarguerita Fontana, DDS

Keywords: Remineralization, Confocal microscopy, NovaMin

Abstract: This study was conducted to evaluate a test dentifrice containing 5% NovaMin and Fluoride (MFP) to a commercially available dentifrice in remineralization of subsurface carious lesions in human tooth enamel.

Enamel sections from 18 extracted human teeth were prepared and sectioned into quadrants. In three sections simulated pre-carius surface lesions a depth of 100-150 µm were created using a demineralizing solution. One quadrant from each tooth was treated with an experimental NovaMin (5%) plus fluoride dentifrice, while another was treated with a commercially available dentifrice with Fluoride (MFP). Each sample (plus an untreated control quadrant) was exposed to 20 days of cycling through demineralizing solution, 3 minute dentifrice treatment, additional demineralizing and then saliva soaking. After treatments the treated quadrants were additionally sectioned to reveal a cross section of the lesion to allow for cross sectional analysis.

Evaluation and measurement was done using confocal laser scanning microscopy ('CLSM'), which is able to distinguish between sound enamel and demineralized enamel using fluorescing dye. The NovaMin dentifrice produced significantly more remineralization than did the Fluoride dentifrice. Fluoride dentifrice® reduced the lesion area by an average of 24.9% from the original lesion depth. NovaMin® decreased the lesion area by 41.9%. Results were statistically significant (p<0.001).

Anti-gingivitis effect of a dentifrice containing bioactive glass (NovaMin ®) particulate

Bao Jun Tai1, Zhuan Bian1,

Han Jiang1, David C. Greenspan2, Jipin Zhong2, Arthur E. Clark3and Min Quan Du1,4

1School of Stomatology, Wuhan University, Wuhan, China; 2NovaMin Technology Inc., Alachua, FL, USA; 3College of Dentistry, University of Florida, Gainesville, FL, USA; 4Key Laboratory of Oral Biomedical Engineering, Wuhan University, Ministry of Education, Wuhan, China

Anti-gingivitis effect of a dentifrice containing bioactive glass (NovaMin ®) particulate. J Clin Periodontol 2006; 33: 86–91. doi: 10.1111/j.1600-051X.2005.00876.x. © Blackwell Munksgaard 2006.

Key words: anti-gingivitis, anti-plaque, bioactive glass.

Abstract

Background: The objective of this pilot clinical trial was to evaluate the anti-gingivitis and anti-plaque effects of a dentifrice containing bioactive glass (NovaMin ®) compared with a placebo control dentifrice in a 6 weeks clinical study.

Methods: The study design was a randomized, double-blinded, controlled clinical trial. One hundred volunteers took part in the study and were matched for plaque index (PLI), gingival bleeding index (GBI), age and gender. The protocol was reviewed and approved by the Ethical Committee of the University. The subjects received a supragingival prophylaxis to remove all plaque, calculus and extrinsic stain. Following the baseline examination, subjects were instructed to brush with their assigned dentifrice and toothbrush. The PLI and GBI were determined for the baseline and 6 weeks. The data were analysed using a repeated-measures ANOVA conducted on the two dependent measures to compare the effect between the test and control group.

Results: Ninety-five subjects finished the study. The results showed that the PLI (baseline = 1.54, 6 weeks = 1.29) and GBI (baseline = 1.14, 6 weeks = 0.47) were significantly reduced, respectively, over the 6 weeks period in the test group (p <0.001 for each measure). There was a 58.8% reduction in gingival bleeding and a 16.4% reduction in plaque growth. There was no difference of the PLI (baseline = 1.60, 6 weeks = 1.57) and GBI (baseline = 1.18, 6-week = 1.02) over the 6 week period in the control group.

Conclusion: This study demonstrated that a dentifrice containing NovaMin significantly improves oral health as measured by a reduction in gingival bleeding and reduction in supragingival plaque compared with a negative dentifrice over the 6 weeks study period.

Tooth Hypersensitivty Reduction by a Novel Bioglass° (NovaMin) Dentifrice

AADR Abstract

Originally Presented at

1998 AADR Meeting

Abstract 747

J.Dent Res;77:199

L.J. Litkowski, K.B. Quinlan and N.J. McDonald

Dental School, University of 7kilarland, Baltimore, MD USA

INTRODUCTION: Tooth hypersensitivity is a condition that affects up to 20% of the adult populationin the U.S. Success with treatment of this condition has been limited at best. A new dentifrice containing a modified Bioglass (NovaMin) material replacing part of the abrasive silica has been developed that showed a significant amount of tubule occlusion during in vitro evaluations. The purpose of this study was to evaluate the clinical efficacy of the Bioalass dentifrice in reducing tooth hypersensitivity.

MATERIALS AND METHODS: Patients were screened for cervical dentin hypersensitivity by utilizing a standard cold air blast and a standard tactile stimulus using a Yeagle Probe with patients rating the pain on a Visual Analog Scale (VAS) from 0 to 100 mm. 66 patients were selected withVAS scores between 30 and 70 mm and divided into 3 groups, placebo (0%), 2.5943 and 7.5°,43 active ingredients. Each patient was told to brush twice per day and was evaluated at 2, 4 and 8 weeks. The scores were tabulated and analyzed using an ANOVA.

RESULTS: Reduction of sensitivity versus baseline is shown in the chart below:

Mean Changes from Baseline

*significantly different from the placebo p<.05, **significantly different from the placebo p‹.01

A significant reduction in tooth hypersensitivity was seen in patients using 2.5% and 7.5°/0 concentrations of Bioglass dentifrice. The 7.5°/0 showed a more rapid and greater magnitude of reduction in sensitivity.

This study was partially supported by US Biomaterials Corporation